Expression, purification, crystallization and preliminary crystallographic analysis of the calponin-homology domain of Rng2

Academic Article

Abstract

  • Rng2 is a multidomain protein component of the actiomyosin ring and the spindle pole body necessary for cytokinesis in Schizosaccharomyces pombe. The calponin-homology domain of Rng2 from S. pombe has been overexpressed, purified and crystallized. The crystals belong to space group P21. Br- and Hg-derivative data sets were measured to 2.21 Å using synchrotron radiation from crystals that were partially fixed with glutaraldehyde. Electron-density maps have been obtained from two-wavelength MAD on the Br derivative and SAD on the Hg derivative.
  • Authors

    Digital Object Identifier (doi)

    Author List

  • Wang CH; Walsh M; Balasubramanian MK; Dokland T
  • Start Page

  • 1809
  • End Page

  • 1812
  • Volume

  • 59
  • Issue

  • 10