Some evidence in the spirituality literature suggests that one's spirituality and religiousness may increase in response to aging and coping with a serious illness. This study examined differences in spirituality in 201 older adults (50 years old or older) and younger adults (21-49 years old) with and without HIV, and examined the biopsychosocial correlates of spirituality. The Ironson-Woods Spirituality/Religiousness Index was used to measure various aspects of spirituality levels; the biopsychosocial measures included the Profile of Mood States, the Lubben Social Network Scale, and a general health questionnaire. Results indicated that the four groups did not differ significantly on measures of spirituality or religiousness. However, in the HIV-positive group, those who were more spiritual or religious had larger social networks, better overall mood, better self-rated health, and had fewer medical conditions. As people continue to age with HIV thanks to advances in pharmaceuticals, spirituality and religiousness may be important qualities to facilitate successful aging in this emerging and growing population. © Taylor & Francis Group, LLC.