Crystallization and preliminary crystallographic investigation of porcine quinolinate phosphoribosyltransferase

Academic Article

Abstract

  • Quinolinate phosphoribosyltransferase (QPRT), purified from hog liver, has been crystallized using PEG 8000 as the precipitant. The crystals form long hexagonal rods in the space group P6322 with cell dimensions a = b = 121.7, c = 94.5 Å. Based on the unit-cell dimensions and the calculated molecular mass of 33 500 Da, the Matthews coefficient suggests one molecule per asymmetric unit (V(m) = 3.45 Å3 Da-1; 64% solvent). Three native data sets were collected to a resolution of 2.5 Å and merged to provide a set that is 94.7% complete, with an R(sym) value of 9.6%.
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    Author List

  • Moore D; Bice T; Jin L; Grandhi A; Delucas LJ; Narayana SVL
  • Start Page

  • 119
  • End Page

  • 120
  • Volume

  • 54
  • Issue

  • 1