Large-scale preparation of the Δ10 form of staphylokinase by in vitro processing of recombinant staphylokinase with purified human plasminogen

Academic Article

Abstract

  • The authors have developed a rapid and convenient method for purification of a low molecular weight form (Δ10) of the bacterial plasminogen activator, staphylokinase. Recombinant staphylokinase is expressed in Escherichia coli, with an amino terminal extension that facilitated purification by immobilized metal-affinity chromatography. Purified staphylokinase is treated with human plasminogen, and the resulting truncated form is purified using a combination of immobilized metal affinity chromatography and hydrophobic interaction chromatography. Purified protein is characterized by amino terminal sequencing and in vitro plasminogen activation assay.
  • Digital Object Identifier (doi)

    Author List

  • Chattopadhyay D; Stewart JE; Delucas LJ
  • Start Page

  • 147
  • End Page

  • 156
  • Volume

  • 69
  • Issue

  • 3