In vitro biochemical assays to monitor rhodopsin function

Academic Article

Abstract

  • Rhodopsin is the dim-light photoreceptor responsible for initiation of the visual transduction cascade. In the dark its activity is very low, while light activation catalyzes the activation of its G-protein transducin. The first step in resetting rhodopsin and the phototransduction cascade involves the phosphorylation of light-active rhodopsin by rhodopsin kinase. Here, we describe assays to monitor the function of rhodopsin or rhodopsin mutants. © 2012 Springer Science+Business Media, LLC.
  • Published In

    Keywords

  • Phototransduction, Rhodopsin, Rhodopsin kinase assay, Rod outer segment preparation, Transducin assay
  • Digital Object Identifier (doi)

    Author List

  • Sammons J; Gross AK
  • Start Page

  • 167
  • End Page

  • 181
  • Volume

  • 884