Nucleotide bound to rab11a controls localization in rod cells but not interaction with rhodopsin

Academic Article

Abstract

  • © 2014 the authors. Precise vectorial transport of rhodopsin is essential for rod photoreceptor health and function. Mutations that truncate or extend the C terminus of rhodopsin disrupt this transport, and lead to retinal degeneration and blindness in human patients and in mouse models. Here we show that such mutations disrupt the binding of rhodopsin to the small GTPase rab11a. The rhodopsin-rab11a interaction is a direct binding interaction that does not depend on the nucleotide binding state of rab11a. Expression of EGFP-rab11a fusion proteins in Xenopus laevis photoreceptors revealed that the nucleotide binding status of rab11a affects its subcellular localization, with GTP-locked mutants concentrated in the inner segment and GDP-locked mutants concentrated in the outer segment. shRNA-mediated knockdown of rab11a in rods led to shortened outer segments and retinal degeneration. Together, our results show the critical importance of direct rhodopsin-rab11a interactions for the formation and maintenance of vertebrate photoreceptors.
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Reish NJ; Boitet ER; Bales KL; Gross AK
  • Start Page

  • 14854
  • End Page

  • 14863
  • Volume

  • 34
  • Issue

  • 45