Caspase-1 Is Not Required for Type 1 Diabetes in the NOD Mouse

Academic Article

Abstract

  • Interleukin (IL)-1β and IL-18 are two cytokines associated with the immunopathogenesis of diabetes in NOD mice. Both of these cytokines are cleaved by caspase-1 to their biologically active forms. IL-1 is a proinflammatory cytokine linked to β-cell damage, and IL-18 stimulates production of interferon (IFN)γ in synergy with IL-12. To examine the effects produced by caspase-1 deficiency on diabetes development in NOD/Lt mice, a disrupted Casp1 gene was introduced by a speed congenic technique. Casp1-/- bone marrow-derived macrophages stimulated with lipopolysaccharide produced no detectable IL-18, fourfold lower IL-1β, and 20-30% less IL-1α than macrophages from wild-type Casp1+/+ or Casp1+/- controls. Unexpectedly, despite reduced IL-1 and IL-18, there was no change in the rate of diabetes or in total incidence as compared with that in wild-type NOD mice. IL-1 reportedly makes an important pathological contribution in the multidose streptozotocin model of diabetes; however, there was no difference in sensitivity to streptozotocin between NOD mice and NOD.Casp1-/- mice at 40 mg/kg body wt or at 25 mg/kg body wt dosage levels. These findings show that caspase-1 processing of IL-1β and IL-18 is not absolutely required for mediation of spontaneous or chemically induced diabetes pathogenesis in the NOD mouse.
  • Authors

    Published In

  • Diabetes  Journal
  • Digital Object Identifier (doi)

    Author List

  • Schott WH; Haskell BD; Tse HM; Milton MJ; Piganelli JD; Choisy-Rossi CM; Reifsnyder PC; Chervonsky AV; Leiter EH
  • Start Page

  • 99
  • End Page

  • 104
  • Volume

  • 53
  • Issue

  • 1