31P NMR of mammalian cells encapsulated in alginate gels utilizing a new phosphate‐free perfusion medium

Academic Article

Abstract

  • A simple technique permitting long term 31P magnetic resonance spectroscopy of cultured mammalian cells is described. Cells are encapsulated in calcium alginate gels and perfused with a new phosphate‐free medium designed for maintenance of high cell viability. Intracellular pH and β‐NTP/Pi ratios, studied in three cell types, remained stable over a 16–20 h NMR monitoring period. The techniques described may be useful for monitoring cell metabolism with a variety of cell types. Copyright © 1990 John Wiley & Sons, Ltd.
  • Published In

  • NMR in Biomedicine  Journal
  • Digital Object Identifier (doi)

    Author List

  • Narayan KS; Moress EA; Chatham JC; Barker PB
  • Start Page

  • 23
  • End Page

  • 26
  • Volume

  • 3
  • Issue

  • 1