Expression of the human neutrophil elastase gene: positive and negative transcriptional elements in the 5' flanking region.

Academic Article

Abstract

  • The structure and position of cis-acting DNA sequences which regulate tissue specific expression of the human neutrophil elastase (HNE) gene have been investigated. We have identified a positive and a negative regulatory element upstream from the promoter region. The ability of these sequences to regulate transcription in myeloid and non-myeloid cells was studied by inserting varying lengths of HNE 5'-flanking sequence into a reporter plasmid containing the bacterial chloramphenicol acetyltransferase (CAT) gene. CAT activity in U937 was minimal in the absence of promoter and in the presence of HNE sequence to -102 bp. Inclusion of sequence up to -153 bp resulted in a 5.6-fold increase in CAT activity that was not observed in non-myeloid transfectants. Extension of the insert to include additional HNE sequence to -196 bp resulted in a decrease in CAT activity to control levels.
  • Authors

    Keywords

  • Base Sequence, Cell Line, Chloramphenicol O-Acetyltransferase, Chromosome Deletion, Gene Expression Regulation, Enzymologic, Genetic Vectors, Humans, Leukocyte Elastase, Molecular Sequence Data, Oligodeoxyribonucleotides, Pancreatic Elastase, Peroxidase, Plasmids, Polymerase Chain Reaction, Recombinant Fusion Proteins, Regulatory Sequences, Nucleic Acid, Sequence Homology, Nucleic Acid, Transcription, Genetic, Transfection
  • Author List

  • Han J; Unlap T; Rado TA
  • Start Page

  • 1462
  • End Page

  • 1468
  • Volume

  • 181
  • Issue

  • 3