Low temperature induces the delivery of mature and immature CFTR to the plasma membrane

Academic Article

Abstract

  • Deletion of phenylalanine 508 (ΔF508) is the most prevalent disease-causing mutation resulting in retention of the immature CFTR in the endoplasmic reticulum. The most common strategy to induce the delivery of ΔF508-CFTR to the surface of cells is by reducing the incubation temperature (≈28 °C). Cell surface biotinylation of HEK293T cells grown at 37 °C for 48 h, confirmed the presence of mature wild-type CFTR, but not ΔF508-CFTR at the cell surface. On the other hand, cells incubated at 28 °C for 16 h showed both mature and immature ΔF508-CFTR at their surface. The trafficking of immature ΔF508-CFTR, but not mature ΔF508-CFTR, to the cell surface occurred at low temperature even upon addition of BFA, suggesting the involvement of a Golgi-independent pathway. These results suggest that low temperature induces the appearance of a mix population of mature and immature CFTR molecules at the plasma membrane through distinct pathways. © 2007 Elsevier Inc. All rights reserved.
  • Digital Object Identifier (doi)

    Author List

  • Rennolds J; Boyaka PN; Bellis SL; Cormet-Boyaka E
  • Start Page

  • 1025
  • End Page

  • 1029
  • Volume

  • 366
  • Issue

  • 4