Arachidonic acid induces an increase in the cytosolic calcium concentration in single pancreatic islet beta cells

Academic Article

Abstract

  • The insulin secretagogue D-glucose induces both accumulation of nonesterified arachidonic acid (35 μM) in pancreatic islets and a rise in beta cell cytosolic [Ca ++ ] i . Arachidonate amplifies both voltage-dependent Ca ++ entry in secretory cells and depolarization-induced insulin secretion. Here, arachidonate induced a biphasic rise in [Ca ++ ] i of Fura-2AM loaded beta cells which increased with arachidonate concentration (5-30 μM), was reversed upon washout, and was unaffected by the arachidonate oxygenase inhibitor BW755C. The sustained phase of the rise was abolished by removal of extracellular Ca ++ and amplified by depolarization with KCl. The accumulation of nonesterified arachidonate in islets stimulated by D-glucose may therefore promote the D-glucose-induced rise in beta cell [Ca ++ ] i . © 1992.
  • Digital Object Identifier (doi)

    Author List

  • Ramanadham S; Gross R; Turk J
  • Start Page

  • 647
  • End Page

  • 653
  • Volume

  • 184
  • Issue

  • 2