Heterotrimeric kinesin-2 (KIF3) mediates transition zone and axoneme formation of mouse photoreceptors.

Academic Article

Abstract

  • Anterograde intraflagellar transport (IFT) employing kinesin-2 molecular motors has been implicated in trafficking of photoreceptor outer segment proteins. We generated embryonic retina-specific (prefix "emb") and adult tamoxifen-induced (prefix "tam") deletions of KIF3a and IFT88 in adult mice to study photoreceptor ciliogenesis and protein trafficking. In (emb)Kif3a(-/-) and in (emb)Ift88(-/-) mice, basal bodies failed to extend transition zones (connecting cilia) with outer segments, and visual pigments mistrafficked. In contrast, (tam)Kif3a(-/-) and (tam)Ift88(-/-) photoreceptor axonemes disintegrated slowly post-induction, starting distally, but rhodopsin and cone pigments trafficked normally for more than 2 weeks, a time interval during which the outer segment is completely renewed. The results demonstrate that visual pigments transport to the retinal outer segment despite removal of KIF3 and IFT88, and KIF3-mediated anterograde IFT is responsible for photoreceptor transition zone and axoneme formation.
  • Published In

    Keywords

  • IFT88, KIF3A, anterograde intraflagellar transport (IFT), cilia, conditional IFT88 knockout, conditional KIF3A knockout, gene knockout, heterotrimeric kinesin-2, homologous recombination, intraflagellar transport, photoreceptor, retinal degeneration, Animals, Axoneme, Basal Bodies, Kinesin, Mice, Mice, Knockout, Protein Transport, Retinal Cone Photoreceptor Cells, Rhodopsin, Tumor Suppressor Proteins
  • Digital Object Identifier (doi)

    Author List

  • Jiang L; Wei Y; Ronquillo CC; Marc RE; Yoder BK; Frederick JM; Baehr W
  • Start Page

  • 12765
  • End Page

  • 12778
  • Volume

  • 290
  • Issue

  • 20