Perinatal taurine depletion followed by high sugar intake (postweaning) alters the renin-angiotensin system (RAS) and glucose regulation in adult female rats. This study tests the hypothesis that in adult female rats, RAS and estrogen contribute to insulin resistance resulting from perinatal taurine imbalance. Female Sprague-Dawley rats were fed normal rat chow with 3% b -alanine (taurine depletion, TD), 3% taurine (taurine supplementation, TS), or water alone (control, C) from conception to weaning. Their female offspring were fed normal rat chow with 5% glucose in water (TDG, TSG, CG) or water alone (TDW, TSW, CW) throughout the experiment. At 7-8 weeks of age, animals were studied with or without captopril inhibition of the RAS and with or without estrogen receptor inhibition by tamoxifen. Compared to CW and CG groups, perinatal taurine depletion but not supplementation slightly increased plasma insulin levels. High sugar intake slightly increased plasma insulin only in TSG. Captopril treatment significantly increased plasma insulin in all groups except CG (the greatest increase was in TDG). Changes in insulin resistance and insulin secretion paralleled the changes in plasma insulin levels. In contrast, tamoxifen treatment increased insulin resistance and decreased insulin secretion only in TDG and this group displayed hyperglycemia and glucose intolerance. These data indicate that perinatal taurine imbalance alters the interplay of RAS and estrogen on glucose-insulin regulation in adult female rats. © Springer Science+Business Media New York 2013.