Heteromeric assembly of acid-sensitive ion channel and epithelial sodium channel subunits.

Academic Article

Abstract

  • Amiloride-sensitive ion channels are formed from homo- or heteromeric combinations of subunits from the epithelial Na+ channel (ENaC)/degenerin superfamily, which also includes the acid-sensitive ion channel (ASIC) family. These channel subunits share sequence homology and topology. In this study, we have demonstrated, using confocal fluorescence resonance energy transfer microscopy and co-immunoprecipitation, that ASIC and ENaC subunits are capable of forming cross-clade intermolecular interactions. We have also shown that combinations of ASIC1 with ENaC subunits exhibit novel electrophysiological characteristics compared with ASIC1 alone. The results of this study suggest that heteromeric complexes of ASIC and ENaC subunits may underlie the diversity of amiloride-sensitive cation conductances observed in a wide variety of tissues and cell types where co-expression of ASIC and ENaC subunits has been observed.
  • Published In

    Keywords

  • Acid Sensing Ion Channels, Animals, CHO Cells, Cations, Cricetinae, Cricetulus, Epithelial Sodium Channels, Fluorescence Resonance Energy Transfer, Gene Expression, Humans, Membrane Proteins, Microscopy, Confocal, Nerve Tissue Proteins, Organ Specificity, Protein Subunits, Sequence Homology, Amino Acid, Sodium Channels
  • Digital Object Identifier (doi)

    Author List

  • Meltzer RH; Kapoor N; Qadri YJ; Anderson SJ; Fuller CM; Benos DJ
  • Start Page

  • 25548
  • End Page

  • 25559
  • Volume

  • 282
  • Issue

  • 35