High-temporal-resolution analysis demonstrates that ICAM-1 stabilizes WEHI 274.1 monocytic cell rolling on endothelium.

Academic Article


  • Leukocyte rolling, adhesion, and migration on vascular endothelium involve several sets of adhesion molecules that interact simultaneously. Each of these receptor-ligand pairs may play multiple roles. We examined the role of ICAM-1 in adhesive interactions with mouse aortic endothelial cells (MAECs) in an in vitro flow system. Average rolling velocity of the monocytic cell line WEHI 274.1 was increased on ICAM-1-deficient MAECs compared with wild-type MAECs, both with and without TNF-alpha stimulation. High-temporal-resolution analysis provided insights into the underlying basis for these differences. Without TNF-alpha stimulation, average rolling velocity was slower on wild-type than on ICAM-1-deficient endothelium because of brief (<1 s) pauses. On TNF-alpha-stimulated ICAM-1-deficient endothelium, cells rolled faster because of transient accelerations, producing "jerky" rolling. Firm adhesion to ICAM-1-deficient MAECs was significantly reduced compared with wild-type MAECs, although the number of rolling cells was similar. These results demonstrate directly that ICAM-1 affects rolling velocity by stabilizing leukocyte rolling.
  • Authors


  • Animals, Antineoplastic Agents, Aorta, Cell Adhesion, Cell Communication, Cells, Cultured, Endothelium, Vascular, Intercellular Adhesion Molecule-1, Mice, Mice, Mutant Strains, Microscopy, Video, Monocytes, P-Selectin, Tumor Necrosis Factor-alpha
  • Digital Object Identifier (doi)

    Author List

  • Kevil CG; Chidlow JH; Bullard DC; Kucik DF
  • Start Page

  • C112
  • End Page

  • C118
  • Volume

  • 285
  • Issue

  • 1