2D difference gel electrophoresis of prepubertal and pubertal rat mammary gland proteomes.

Academic Article

Abstract

  • Rat mammary gland proteomes at day 21 (prepubertal) and day 50 (late puberty) were compared by 2D difference gel electrophoresis. Two-hundred fifty-one spots were significantly different ( p < 0.05) in abundance. Peptide mass fingerprint analysis of a subset of these proteins identified two significantly over-represented classes including structural and blood proteins (increased), and metabolism-relevant proteins (reduced) in day 50 relative to day 21 glands. This is a first report of mammary gland proteome differences at these important breast cancer-relevant time-points.
  • Published In

    Keywords

  • Aging, Animals, Carbocyanines, Electrophoresis, Gel, Two-Dimensional, Female, Fluorescent Dyes, Mammary Glands, Animal, Peptide Mapping, Proteome, Rats, Rats, Sprague-Dawley, Sexual Maturation, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Digital Object Identifier (doi)

    Author List

  • Kim H; Cope MB; Herring R; Robinson G; Wilson L; Page GP; Barnes S
  • Start Page

  • 4638
  • End Page

  • 4650
  • Volume

  • 7
  • Issue

  • 11