Development of dermatitis in CD18-deficient PL/J mice is not dependent on bacterial flora, and requires both CD4+ and CD8+ T lymphocytes

Academic Article


  • CD18-deficient PL/J mice develop dermatitis characterized by hyperkeratosis, and a mixed dermal and epidermal inflammatory infiltrate. The development of this disease requires low-level CD18 expression and at least two PL/J loci. Currently, the mechanisms by which decreased β2 integrin expression on leukocytes promotes skin inflammation in PL/J mice are unknown. In these studies, we investigated the role of microbial infection and T lymphocytes in the pathogenesis of this disease. We found that germ-free CD18-/- PL/J mice developed dermatitis indistinguishable from that of mice raised in pathogen-free conditions. Adoptive transfer of CD18-/- PL/J splenocytes into skin disease-resistant CD18+/- PL/J mice failed to induce skin inflammation. However, transfer of CD18+/- splenocytes blocked the progression and ultimately led to resolution of skin disease in the majority of CD18-/- recipients. Depletion of both CD4+ and CD8+ T cells mice prior to onset of the disease significantly delayed the appearance of inflammatory skin disease. In contrast, single depletions of these T cells did not inhibit disease development. These studies show that dermatitis in CD18-deficient PL/J mice is not the consequence of infection, does not require bacterial superantigens, and is mediated by both CD4+ and CD8+ T lymphocytes. Furthermore, they suggest that one possible mechanism for skin disease development in these mice may involve the absence or dysfunctional activity of a regulatory T cell population. These mice may therefore be useful in identifying potential mechanisms of pathogenesis and genetic predisposition in human inflammatory skin diseases.
  • Published In

    Digital Object Identifier (doi)

    Pubmed Id

  • 25689495
  • Author List

  • Barlow SC; Xu H; Weaver CT; Lindsey JR; Schoeb TR; Bullard DC
  • Start Page

  • 345
  • End Page

  • 351
  • Volume

  • 16
  • Issue

  • 2