Proper coronary vascular development and heart morphogenesis depend on interaction of GATA-4 with FOG cofactors

Academic Article


  • GATA-family transcription factors are critical to the development of diverse tissues. In particular, GATA-4 has been implicated in formation of the vertebrate heart. As the mouse Gata-4 knock-out is early embryonic lethal because of a defect in ventral morphogenesis, the in vivo function of this factor in heart development remains unresolved. To search for a requirement for Gata4 in heart development, we created mice harboring a single amino acid replacement in GATA-4 that impairs its physical interaction with its presumptive cardiac cofactor FOG-2. Gata4ki/ki mice die just after embryonic day (E) 12.5 exhibiting features in common with Fog2-/- embryos as well as additional semilunar cardiac valve defects and a double-outlet right ventricle. These findings establish an intrinsic requirement for GATA-4 in heart development. We also infer that GATA-4 function is dependent on interaction with FOG-2 and, very likely, an additional FOG protein for distinct aspects of heart formation.
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    Digital Object Identifier (doi)

    Author List

  • Crispino JD; Lodish MB; Thurberg BL; Litovsky SH; Collins T; Molkentin JD; Orkin SH
  • Start Page

  • 839
  • End Page

  • 844
  • Volume

  • 15
  • Issue

  • 7