Notch2 haploinsufficiency results in diminished B1 B cells and a severe reduction in marginal zone B cells

Academic Article


  • Recent studies have implicated a role for Notch in the generation of marginal zone (MZ) B cells. To further investigate the role of Notch in the B cell lineage, we have analyzed the effects of reduced Notch2 signaling in mice expressing one functional allele of Notch2 (Notch2+/-). Notch2 +/- mice have reduced B1 B cells of the peritoneal cavity and show a severe reduction in MZ B cells of the spleen. The reduction in MZ B cells was not due to the disruption of splenic architecture, disregulated terminal differentiation, nor to increased apoptosis within the MZ B cell compartment. Rather, our data suggest that Notch2 haploin-sufficiency leads to impaired development of MZ B cells, possibly by impacting the formation of immediate MZ B precursors. These results provide evidence that Notch2 plays a determining role in the development and/or the maintenance of B1 B and MZ B cells.
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Witt CM; Won WJ; Hurez V; Klug CA
  • Start Page

  • 2783
  • End Page

  • 2788
  • Volume

  • 171
  • Issue

  • 6