An embryonic source of Ly1 but not conventional B cells.

Academic Article

Abstract

  • Ly1+ B cells differ from conventional B cells with respect to their anatomical localization, cell surface marker expression, and antibody repertoire suggesting that they may constitute a functionally distinct subset of B cells. To determine whether Ly1+ B cells also have a developmentally distinct site of origin we grafted various fetal primordia into adult severe combined immunodeficient (scid) mice and analyzed their potential to give rise to T and B cells. We demonstrated that fetal omentum, but not spleen or thymus grafts, reconstituted exclusively Ly1 B cells (including the Ly1 sister population) as well as a population of IgM and IgA producing plasma cells in the spleen and gut, respectively. Although thymus grafts regularly reconstituted T cells, thymus plus fetal omentum cografts gave rise to a population of Ly1+ B cells as well as T cells which were also derived from omentum. However, in neither omentum nor omentum plus thymus cografts were conventional B cells detected. These results provide the first evidence that Ly1 B cells but not conventional B cells are generated from the fetal omentum.
  • Published In

    Keywords

  • Animals, Antigens, Ly, B-Lymphocytes, Female, Hematopoietic Stem Cell Transplantation, Hematopoietic Stem Cells, Hybridomas, Immunoglobulin Isotypes, Immunologic Deficiency Syndromes, Male, Mice, Mice, Inbred Strains, Omentum, Plasma Cells, T-Lymphocytes
  • Author List

  • Solvason N; Lehuen A; Kearney JF
  • Start Page

  • 543
  • End Page

  • 550
  • Volume

  • 3
  • Issue

  • 6