Immunofluorescence analysis of B-1 cell ontogeny in the mouse.

Academic Article

Abstract

  • In order to further understand the developmental aspects of B-1 cells, we characterized the ontogeny of this B cell population in the spleen and peritoneal cavity of BALB/c mice. Although there are B-1 cells in the spleen within the first 1-3 weeks after birth, they do not at any stage represent the majority of splenic B cells. Splenic B-1 cells reach peak levels at approximately 9 days after birth. The mesenteric lining that covers the small intestine of 7-day-old mice contains a population of IgM+ B cells, while at the same age, there are few lymphoid cells in the peritoneal cavity. Between 7 and 8 days after birth there is an influx of B cells into the peritoneal cavity. At 8 days, the first detectable peritoneal B cells appear to be of the B-1 type based on expression of IL-5 receptor and CD5. However, these peritoneal B-1 cells do not express Mac-1. This antigen is not expressed by the majority of peritoneal B-1 cells until 3 weeks. This study indicates that the majority of early splenic B cells are not B-1 cells and it suggests that the mesenteric tissues surrounding the gut contain B lymphocytes which traffic into the peritoneal cavity where they then reside.
  • Published In

    Keywords

  • Aging, Animals, Animals, Newborn, Antibodies, Monoclonal, Antigens, CD, B-Lymphocyte Subsets, CD5 Antigens, Fluorescent Antibody Technique, Immunoglobulin M, Macrophage-1 Antigen, Mesentery, Mice, Mice, Inbred BALB C, Peritoneal Cavity, Peritoneal Lavage, Receptors, Interleukin, Receptors, Interleukin-5, Spleen
  • Author List

  • Hamilton AM; Lehuen A; Kearney JF
  • Start Page

  • 355
  • End Page

  • 361
  • Volume

  • 6
  • Issue

  • 3