Development of VH81X transgene-bearing B cells in fetus and adult: sites for expansion and deletion in conventional and CD5/B1 cells.

Academic Article

Abstract

  • The most D-proximal functional VH gene, VH81X, is preferentially expressed in the mouse fetal B cell repertoire; however, it is expressed in few B cells in the adult. To determine when VH81X gene expression affects size and phenotype of particular stages in B cell differentiation, transgenic mice have been developed expressing a germline fetal liver-derived VH81X-mu rearrangement. Comparative analysis of B lymphopoiesis reveals similarities and differences between fetal liver and adult bone marrow which pinpoint developmental stages in mice during which VH81X-expressing B cell progenitors expand or deplete compartment sizes. These include a similar reduction in c-kitR+ and establishment of a predominant CD43low/HSAhigh phenotype within the B220+ CD43+ compartment which is dependent on the association of the transgene with lambda 5. In contrast, the CD43- pre-B and immature B cell compartments are expanded in the fetus but not in the adult. In addition, there are other factors that later disfavor the survival of VH81X-expressing B1 and B2 cells. Thus the failure to detect VH81X-bearing B cells in the adult is the result of a multistep selection process occurring at all stages during B repertoire expansion.
  • Published In

    Keywords

  • Aging, Animals, Animals, Newborn, Antigens, CD, B-Lymphocytes, Bone Marrow, Bone Marrow Cells, Cell Differentiation, Clonal Deletion, Embryonic and Fetal Development, Genes, Immunoglobulin, Hybridomas, Immunoglobulin Heavy Chains, Immunoglobulin Variable Region, Leukosialin, Liver, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Transgenic, Proto-Oncogene Proteins c-kit, Radiation Chimera, Sialoglycoproteins, Transgenes
  • Author List

  • Martin F; Chen X; Kearney JF
  • Start Page

  • 493
  • End Page

  • 505
  • Volume

  • 9
  • Issue

  • 4