Marginal zone B cells exhibit unique activation, proliferative and immunoglobulin secretory responses.

Academic Article

Abstract

  • Mouse splenic B cells can be separated based on their distinctive expression of cell surface antigens. Marginal zone (MZ) B cells are CD38high CD21high CD23low/-, while follicular (FO) B cells are CD21int CD23int and newly formed (NF) B cells are CD21dim/- CD23-. Exploiting these phenotypic distinctions, we isolated the three B cell subsets and assessed their other phenotypic differences and functional capabilities in vitro. FO B cells proliferate more than the other B cell subsets in response to either IgM or CD38 cross-linking. MZ B cells proliferate better than FO B cells when stimulated with lipopolysaccharide (LPS), sub-optimal levels of LPS and CD38 cross-linking or CD40 ligation. When NF, FO and MZ B cells were stimulated with either LPS or LPS and interleukin-4, MZ B cells secreted more IgM and IgG3 than the other two subsets. Similarly, calcium fluxes measured within MZ B cells are larger in amplitude and more sustained after B cell receptor cross-linking than those found in the other two subsets. Collectively, these results indicate that CD38high CD21high CD23low/- MZ B cells are specially suited to play a unique role in response to antigens delivered to the MZ area.
  • Published In

    Keywords

  • Animals, B-Lymphocytes, Calcium, Cell Cycle, Gene Expression, Genes, Immunoglobulin, Immunoglobulins, Lymphocyte Activation, Lymphocyte Subsets, Lymphocytes, Mice, Mice, Inbred BALB C, Mice, Inbred DBA, Proto-Oncogene Proteins c-bcl-2, RNA, Messenger, Spleen
  • Digital Object Identifier (doi)

    Author List

  • Oliver AM; Martin F; Gartland GL; Carter RH; Kearney JF
  • Start Page

  • 2366
  • End Page

  • 2374
  • Volume

  • 27
  • Issue

  • 9