Increased junctional diversity in fetal B cells results in a loss of protective anti-phosphorylcholine antibodies in adult mice.

Academic Article

Abstract

  • Fetal Igs are less diverse than adult Igs, largely because of the lack of N addition in the absence of Tdt. To test whether the absence of Tdt is essential, we generated Tg mice that express Tdt and add N regions in fetal B cells. When challenged as adults with PC-containing Streptococcus pneumoniae, these mice fail to make the hallmark T15 anti-PC Ab encoded by canonical rearrangements of Ig H and L chain genes. The anti-PC Abs from these mice are altered by premature N addition and do not protect against death from virulent pneumococcal infection. These results show that maintenance of lower Ig diversity in early life is essential for the acquisition of a complete functional adult repertoire.
  • Published In

  • Immunity  Journal
  • Keywords

  • Animals, Antibodies, Antibody Diversity, DNA Nucleotidylexotransferase, Fetus, Gene Rearrangement, Gene Rearrangement, B-Lymphocyte, Genetic Variation, Immunoglobulin Heavy Chains, Immunoglobulin Light Chains, Mice, Mice, Mutant Strains, Molecular Sequence Data, Phosphorylcholine
  • Author List

  • Benedict CL; Kearney JF
  • Start Page

  • 607
  • End Page

  • 617
  • Volume

  • 10
  • Issue

  • 5