Positive selection from newly formed to marginal zone B cells depends on the rate of clonal production, CD19, and btk.

Academic Article

Abstract

  • Using immunoglobulin heavy chain transgenic mice, we show that B cell clones reaching the long-lived pool are heterogeneous: some are enriched in the CD21(high) compartment (mostly marginal zone [MZ]), others reside primarily in the follicles (FO). Altering the composition of the B cell receptor through N region additions decreases the rate of clonal production and the MZ enrichment. This process can be recapitulated by purified CD21(low) B cells and is due to a preferential clonal survival that requires a functional btk tyrosine kinase. We also show that generation of the MZ population is dependent on CD19. These findings suggest that the MZ B cell repertoire is positively selected and have functional implications for antigenic responses effected by B cells from this microenvironment.
  • Published In

  • Immunity  Journal
  • Keywords

  • Agammaglobulinaemia Tyrosine Kinase, Animals, Antigens, CD19, B-Lymphocytes, Bone Marrow Cells, Cell Differentiation, Cells, Cultured, Clone Cells, Hematopoietic Stem Cells, Immunoglobulin Idiotypes, Immunophenotyping, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Inbred CBA, Mice, Transgenic, Protein-Tyrosine Kinases, Receptors, Antigen, B-Cell, Receptors, Complement 3d, Receptors, IgE, Spleen
  • Author List

  • Martin F; Kearney JF
  • Start Page

  • 39
  • End Page

  • 49
  • Volume

  • 12
  • Issue

  • 1