Murine marginal zone B cells play a role in Vibrio cholerae LPS antibody responses

Academic Article

Abstract

  • The emergence of Vibrio cholerae (Vc) lipopolysaccharide (LPS) as a lead protective antigen for a cholera subunit vaccine has increased the interest in what type of B cell is best suited to generate anti-Vc LPS antibodies. A related question is what form of LPS is the most immunogenic. C57Bl/6 (B6) neonatal mice (10¬†days old) whose marginal zone (MZ) B cell compartment is still maturing and two lines of knockout mice that either lack the signaling mechanism required for the maturation of MZ B cells or that lack a receptor required for MZ B cell retention in the MZ were used to determine the role of MZ B cells in anti-Vc LPS antibody responses. Data support the conclusion that MZ B cells play a significant role in the anti-Vc LPS antibody response. Serum and vibriocidal antibody titers also depend on whether the Vc LPS is purified or bacterial cell-associated. New perspectives are needed to build a universal cholera vaccine. ¬© 2013 Federation of European Microbiological Societies.
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    Digital Object Identifier (doi)

    Author List

  • Wade WF; King RG; Grandjean C; Wade TK; Justement LB
  • Start Page

  • 153
  • End Page

  • 157
  • Volume

  • 70
  • Issue

  • 2