Purification, crystallization and X-ray analysis of Hibiscus chlorotic ringspot virus.

Academic Article

Abstract

  • Hibiscus chlorotic ringspot virus (HCRSV), a Carmovirus, occurs worldwide and induces chlorotic ringspots on leaves, stunting and flower distortion in Hibiscus species, including kenaf. The HCRSV capsid has T = 3 icosahedral symmetry and contains 180 copies of the coat protein. A virus yield of 48-70 mg per 100 g of infected kenaf leaves was achieved with an improved purification scheme involving sucrose-cushion and sucrose density-gradient centrifugation. The virus was crystallized using PEG 8000 and 2,3-butanediol as co-precipitants. The crystals belonged to the cubic space group P23, with unit-cell parameter a = 392 A, and diffracted X-rays to at least 4.5 A resolution.
  • Authors

    Keywords

  • Butylene Glycols, Carmovirus, Centrifugation, Density Gradient, Crystallization, Crystallography, X-Ray, Microscopy, Electron, Oscillometry, Plant Leaves, Polyethylene Glycols, X-Ray Diffraction
  • Author List

  • Lee K-C; Lim D; Wong S-M; Dokland T
  • Start Page

  • 1481
  • End Page

  • 1483
  • Volume

  • 59
  • Issue

  • Pt 8