Grape exosome-like nanoparticles induce intestinal stem cells and protect mice from DSS-induced colitis.

Academic Article

Abstract

  • Food-derived exosome-like nanoparticles pass through the intestinal tract throughout our lives, but little is known about their impact or function. Here, as a proof of concept, we show that the cells targeted by grape exosome-like nanoparticles (GELNs) are intestinal stem cells whose responses underlie the GELN-mediated intestinal tissue remodeling and protection against dextran sulfate sodium (DSS)-induced colitis. This finding is further supported by the fact that coculturing of crypt or sorted Lgr5⁺ stem cells with GELNs markedly improved organoid formation. GELN lipids play a role in induction of Lgr5⁺ stem cells, and the liposome-like nanoparticles (LLNs) assembled with lipids from GELNs are required for in vivo targeting of intestinal stem cells. Blocking β-catenin-mediated signaling pathways of GELN recipient cells attenuates the production of Lgr5⁺ stem cells. Thus, GELNs not only modulate intestinal tissue renewal processes, but can participate in the remodeling of it in response to pathological triggers.
  • Authors

    Published In

  • Molecular Therapy  Journal
  • Keywords

  • Animals, Colitis, Dextran Sulfate, Intestines, Male, Mice, Nanoparticles, Stem Cells, Vitis
  • Digital Object Identifier (doi)

    Author List

  • Ju S; Mu J; Dokland T; Zhuang X; Wang Q; Jiang H; Xiang X; Deng Z-B; Wang B; Zhang L
  • Start Page

  • 1345
  • End Page

  • 1357
  • Volume

  • 21
  • Issue

  • 7