Lineage and stage specificity of isotype switching in humans.

Academic Article

Abstract

  • The lineage and stage specificity of human isotype switch recombination was investigated by examining the IgH gene configuration in 61 hemopoietic malignancies representing different stages of B and T cell development. An unexpectedly high frequency (20%) of IgM-producing B cell leukemias and lymphomas had undergone CH gene rearrangements and deletions consistent with attempted switch recombination. These CH gene alterations were found on productive, non-productive, and 14q+ chromosomes. These data support the concept of a non-specific (common) switch recombinase activity that is often ineffective. No evidence of such switch recombination was found in 25 mu- or mu+ pre-B cell leukemias with the single exception of a mu- pre-B leukemia in which subsets of the cells were producing gamma- or alpha-H chains. The switch recombinase activity gamma- or alpha-H chains. The switch recombinase activity may be restricted to the B cell lineage, inasmuch as CH gene deletions were not observed in T lineage malignancies.
  • Authors

    Published In

    Keywords

  • Cell Transformation, Neoplastic, Chromosome Deletion, Gene Rearrangement, B-Lymphocyte, Hematopoietic Stem Cells, Humans, Immunoglobulin Constant Regions, Immunoglobulin Heavy Chains, Immunoglobulin Switch Region, Leukemia, B-Cell, Lymphocyte Activation, Precursor B-Cell Lymphoblastic Leukemia-Lymphoma, Recombination, Genetic, T-Lymphocytes, Translocation, Genetic
  • Author List

  • Borzillo GV; Cooper MD; Bertoli LF; Landay A; Castleberry R; Burrows PD
  • Start Page

  • 3625
  • End Page

  • 3633
  • Volume

  • 141
  • Issue

  • 10