Molecular cloning of the murine BP-1/6C3 antigen: a member of the zinc-dependent metallopeptidase family.

Academic Article

Abstract

  • The BP-1/6C3 antigen is a phosphorylated cell surface glycoprotein that can be identified by monoclonal antibodies on mouse pre-B cells, immature B cells, and certain stromal cell lines from bone marrow. Expression of this antigen is increased in stromal-dependent pre-B cell lines and retrovirally transformed pre-B cells. Expression of the BP-1/6C3 antigen thus correlates with proliferation and transformation of immature B-lineage cells. In this study, we report the isolation and characterization of cDNAs encoding the BP-1/6C3 antigen. Northern blot analysis revealed a major 4.1-kilobase mRNA in all BP-1/6C3+ mouse pre-B lines and in a human pre-B cell line. BP-1/6C3 mRNA was either absent or truncated in BP-1/6C3- cell lines. The cDNA sequence predicts a type II integral membrane protein of 945 amino acids with an intracytoplasmic amino terminus of only 17 amino acids and a typical zinc-binding motif in its extracellular domain. BP-1/6C3 has significant homology to aminopeptidase N and is the second member of the zinc-dependent metallopeptidase gene family to be found on the surface of early B-lineage cells.
  • Authors

    Keywords

  • Amino Acid Sequence, Animals, B-Lymphocytes, Base Sequence, Blotting, Northern, Cell Line, Cell Membrane, Cloning, Molecular, DNA, Gene Library, Humans, Metalloendopeptidases, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Poly A, RNA, RNA, Messenger, Rats, Sequence Homology, Nucleic Acid, Zinc
  • Authorlist

  • Wu Q; Lahti JM; Air GM; Burrows PD; Cooper MD
  • Start Page

  • 993
  • End Page

  • 997
  • Volume

  • 87
  • Issue

  • 3