The human PD-1 gene: complete cDNA, genomic organization, and developmentally regulated expression in B cell progenitors.

Academic Article

Abstract

  • We report the complete cDNA sequence and the genomic structure of the human PD-1 homologue. An analysis of the expression pattern of the human PD-1 gene (hPD-1) and the murine PD-1 gene (mPD-1) in developing bone marrow B-lineage cells was also undertaken. The full length hPD-1 cDNA is 2106 nucleotides long and encodes a predicted protein of 288 amino acid residues. The hPD-1 and mPD-1 genes share 70% homology at the nucleotide level and 60% homology at the amino acid level. Four potential sites for N-linked glycosylation are conserved, as are a stretch of amino acids between two cysteine residues resembling a V-set immunoglobulin domain, and another region containing a motif similar to an immunoreceptor tyrosine-based inhibitory motif. Isolation of the genomic locus of the hPD-1 gene reveals that the gene is composed of five exons located on human chromosome 2 at band q37. The 5' flanking region lacks TATA and CAAT cis-acting elements, but includes a number of potential transcription factor binding sites and a dominant transcription start site. The mPD-1 gene was preferentially expressed in pro-B cells from murine adult bone marrow. Although hPD-1 was not preferentially expressed in pro-B cells from human fetal bone marrow, treatment of isolated pro-B cells with interleukin-7 resulted in a dramatic increase in expression. These data suggest that PD-1 may play a role in B-cell differentiation during the pro-B cell stage.
  • Authors

    Published In

  • Gene  Journal
  • Keywords

  • Amino Acid Sequence, Animals, Antigens, CD, Antigens, Surface, Apoptosis Regulatory Proteins, B-Lymphocytes, Base Sequence, Bone Marrow, Cells, Cultured, Chromosomes, Human, Pair 2, DNA, Complementary, Female, Gene Expression Regulation, Developmental, Genes, Hematopoietic Stem Cells, Humans, Interleukin-7, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Programmed Cell Death 1 Receptor, Proteins, RNA, Messenger, Regulatory Sequences, Nucleic Acid, Restriction Mapping, Sequence Homology, Amino Acid, Sequence Homology, Nucleic Acid
  • Author List

  • Finger LR; Pu J; Wasserman R; Vibhakar R; Louie E; Hardy RR; Burrows PD; Billips LG
  • Start Page

  • 177
  • End Page

  • 187
  • Volume

  • 197
  • Issue

  • 1-2