High throughput sequencing reveals a complex pattern of dynamic interrelationships among human T cell subsets

Academic Article

Abstract

  • Developing T cells face a series of cell fate choices in the thymus and in the periphery. The role of the individual T cell receptor (TCR) in determining decisions of cell fate remains unresolved. The stochastic/selection model postulates that the initial fate of the cell is independent of TCR specificity, with survival dependent on additional TCR/coreceptor "rescue" signals. The "instructive" model holds that cell fate is initiated by the interaction of the TCR with a cognate peptide-MHC complex. T cells are then segregated on the basis of TCR specificity with the aid of critical coreceptors and signal modulators [Chan S, Correia-Neves M, Benoist C, Mathis (1998) Immunol Rev 165: 195-207]. The former would predict a random representation of individual TCR across divergent T cell lineages whereas the latter would predict minimal overlap between divergent T cell subsets. To address this issue, we have used high-throughput sequencing to evaluate the TCR distribution among key T cell developmental and effector subsets from a single donor. We found numerous examples of individual subsets sharing identical TCR sequence, supporting a model of a stochastic process of cell fate determination coupled with dynamic patterns of clonal expansion of T cells bearing the same TCR sequence among both CD4+ and CD8+ populations.
  • Digital Object Identifier (doi)

    Author List

  • Wang C; Sanders CM; Yang Q; Schroeder HW; Wang E; Babrzadeh F; Gharizadeh B; Myers RM; Hudson JR; Davis RW
  • Start Page

  • 1518
  • End Page

  • 1523
  • Volume

  • 107
  • Issue

  • 4