Background. Studies indicate impaired wound healing after trauma. The underlying mechanism remains unknown. Methods. Mice were subjected to midline laparotomy, and polyvinyl alcohol sponges were implanted subcutaneously before hemorrhage (35 ± 5 mmHg for 90 minutes, resuscitated) or sham operation. Wound exudate cells from the sponges were harvested on the first, third, and fifth postoperative day and cultured for 24 hours. Interleukin (IL)-1β, IL-6, monocyte chemotactic protein-1 (MCP-1), macrophage inflammatory protein-2 (MIP-2), and transforming growth factor (TGF)-β were determined in the supernatants. IL-1β and IL-6 were measured in the wound fluid. Results. Hemorrhage decreased collagen deposition in the wound. TGF- β release was significantly decreased on the first and third postoperative days after hemorrhage, whereas IL-1β and IL-6 release was increased at 3 and 5 days after hemorrhage. Similarly, IL-1β and IL-6 in the wound fluid were significantly increased at 3 days after hemorrhage. Conclusions. Because increased levels of pro-inflammatory cytokines and decreased amounts of TGF- β have been reported to impair the process of wound healing, the increased release of IL-1β and IL-6 and the decreased release of TGF-β after hemorrhage might contribute to the decreased collagen production in those animals. Thus, attempts to locally change the ratio of those cytokines in trauma victims might be useful for improving wound healing in those patients.