Triptolide therapy for neuroblastoma decreases cell viability in vitro and inhibits tumor growth in vivo

Academic Article

Abstract

  • Background: Heat shock protein (Hsp)-70 is overexpressed in several human malignancies, and its inhibition has been shown to kill cancer cells. Our objectives were to assess the effectiveness of triptolide, an Hsp-70 inhibitor, in treating neuroblastoma in vitro and in vivo, and to measure the associated effects on Hsp-70 levels and apoptosis markers. Methods: After exposing N2a and SKNSH cell lines to triptolide, cell viability was assessed. Caspase-3 and -9 activities were measured and annexin staining performed to determine if cell death occurred via apoptosis. Hsp-70 protein and mRNA levels were determined using Western blot and real-time polymerase chain reaction. In an orthotopic tumor model, mice received daily triptolide injections and were humanely killed at study completion with tumor measurement. Results: Triptolide treatment resulted in dose- and time-dependent N2a cell death and dose-dependent SKNSH killing. Triptolide exposure was associated with dose-dependent increases in caspase activity and annexin staining. Triptolide decreased Hsp-70 protein and mRNA levels in a dose-dependent fashion. Mice receiving triptolide therapy had significantly smaller tumors than controls. Conclusion: Triptolide therapy decreased neuroblastoma cell viability in vitro and inhibited tumor growth in vivo. Our studies suggest that triptolide killed cells via apoptosis and in association with inhibition of Hsp-70 expression. Triptolide may provide a novel therapy for neuroblastoma. © 2009 Mosby, Inc. All rights reserved.
  • Published In

  • Surgery  Journal
  • Digital Object Identifier (doi)

    Pubmed Id

  • 23683571
  • Author List

  • Antonoff MB; Chugh R; Borja-Cacho D; Dudeja V; Clawson KA; Skube SJ; Sorenson BS; Saltzman DA; Vickers SM; Saluja AK
  • Start Page

  • 282
  • End Page

  • 290
  • Volume

  • 146
  • Issue

  • 2