A noninvasive reporter system to image adenoviral-mediated gene transfer to ovarian cancer xenografts

Academic Article

Abstract

  • Objective. Gene therapy trials for ovarian cancer would benefit from a noninvasive imaging modality to detect the location and extent of gene transfer. The human type 2 somatostatin receptor gene (hSSTr2) was evaluated as a reporter gene for imaging adenoviral (Ad) gene transfer to ovarian cancer. Methods. A replication-incompetent Ad vector encoding hSSTr2 (Ad-hSSTr2) was used to infect SKOV3.ip1 cells in vitro and tumors growing in nude mice. Gamma camera imaging detected uptake of 99m-Tc-P2045 (a somatostatin analogue) due to expressed hSSTr2. Results. Specific uptake of 99m-Tc-P2045 was imaged in Ad-hSSTr2-infected cells in vitro. Noninvasive in vivo imaging detected gene transfer to intraperitoneal tumors. Uptake of 99m-Tc-P2045 (percentage dose per gram of tumor) averaged 2.2 and 0.18 for Ad-hSSTr2-injected mice and controls, respectively. Conclusion. This study reports the first noninvasive imaging method for imaging gene transfer to ovarian cancer. A human gene therapy trial is planned. © 2001 Academic Press.
  • Digital Object Identifier (doi)

    Author List

  • Chaudhuri TR; Rogers BE; Buchsbaum DJ; Mountz JM; Zinn KR
  • Start Page

  • 432
  • End Page

  • 438
  • Volume

  • 83
  • Issue

  • 2