In vitro release of histmaine from murine mast cells by block co-polymers composed of polyoxyethylene and polyoxypropylene

Academic Article

Abstract

  • A series of block co-polymers composed of polyoxyethylene and polyoxypropylene were investigated for their ability to induce in vitro activation of mouse mast cells. We found that six of these copolymers could cause histamine release from mouse mast cells in vitro. At low concentrations, the most efficacious co-polymer, T130R2, caused rapid and extensive concentration-dependent release of histamine from mouse mast cells. The release process was not cytotoxic; it required metabolic energy and was not accompanied by release of lactate dehydrogenase. Optimal release of histamine was dependent on both calcium and sodium ions in the extracellular medium. The degree of in vitro histamine release correlated with in vivo inflammation and in vitro ionophore activity. We believe that this represents the first report of the activation of mediator-containing cells by an ionophore selective for monovalent cations. These copolymers may therefore represent new reagents for investigations of cellular excitation.
  • Authors

    Published In

    Author List

  • Atkinson TP; Smith TF; Hunter RL
  • Start Page

  • 1302
  • End Page

  • 1306
  • Volume

  • 141
  • Issue

  • 4