Determination of gangliosides in six human primary medulloblastomas.

Academic Article

Abstract

  • The ganglioside composition of six human medulloblastomas was analyzed. The characterization was performed by thin-layer chromatography, sialidase hydrolysis, and immunological staining with a panel of characterized antiganglioside monoclonal antibodies. The total ganglioside content ranged from 60 to 1,130 nmol of ganglioside sialic acid/g wet weight. Neuronal gangliosides (gangliotetraose series) were found in varying amounts in all medulloblastomas. Gangliosides of the neolactotetraose series (3'-LM1 and LD1) were present in all specimens, and the lactotetraose series ganglioside 3'-isoLM1 was found in all cases showing astrocytic differentiation. This supports our previous findings that 3'-isoLM1 is a marker for proliferating astroglial cells.
  • Published In

    Keywords

  • Adolescent, Cerebellar Neoplasms, Child, Child, Preschool, Chromatography, Thin Layer, Female, Gangliosides, Humans, Infant, Male, Medulloblastoma, Sialic Acids
  • Author List

  • Gottfries J; Fredman P; MÃ¥nsson JE; Collins VP; von Holst H; Armstrong DD; Percy AK; Wikstrand CJ; Bigner DD; Svennerholm L
  • Start Page

  • 1322
  • End Page

  • 1326
  • Volume

  • 55
  • Issue

  • 4