A rapid microneutralization assay for the measurement of neutralizing antibody reactive with human cytomegalovirus

Academic Article

Abstract

  • We have developed a murine monoclonal antibody reactive with a major immediate early 72 000 dalton protein of human cytomegalovirus and utilized this reagent in a rapid virus titration and microneutralization assay. Because of the early expression of this virus encoded protein, both assays could be accomplished within 16 h following virus inoculation. In addition, both assays resulted in considerable savings of reagents because the assays were carried out in 96-well microtiter plates. These assays should prove useful in the preparation and study of neutralizing antibodies directed against human cytomegalovirus. © 1989.
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Andreoni M; Faircloth M; Vugler L; Britt WJ
  • Start Page

  • 157
  • End Page

  • 167
  • Volume

  • 23
  • Issue

  • 2