Rescue of signaling by a chimeric protein containing the cytoplasmic domain of CD45

Academic Article


  • Surface expression of the CD45 tyrosine phosphatase is essential for the T cell antigen receptor (TCR) to couple optimally with its second messenger pathways. CD45 may be required to dephosphorylate a TCR-activated protein tyrosine kinase, which then transduces an activation signal from the TCR. A chimeric molecule that contained extracellular and transmembrane sequences from an allele of a major histocompatibility class I molecule and cytoplasmic sequences of CD45 restored TCR signaling in a CD45-deficient mutant T cell line. Thus, expression of the complex extracellular domain of CD45 is not required for the TCR to couple to its signaling machinery.
  • Published In

  • Science  Journal
  • Digital Object Identifier (doi)

    Author List

  • Hovis RR; Donovan JA; Musci MA; Motto DG; Goldman FD; Ross SE; Koretzky GA
  • Start Page

  • 544
  • End Page

  • 546
  • Volume

  • 260
  • Issue

  • 5107