The p53/p21WAF/CIPpathway mediates oxidative stress and senescence in dyskeratosis congenita cells with telomerase insufficiency

Academic Article

Abstract

  • Telomere attrition is a natural process that occurs due to inadequate telomere maintenance. Once at a critically short threshold, telomeres signal growth arrest, leading to senescence. Telomeres can be elongated by the enzyme telomerase, which adds de novo telomere repeats to the ends of chromosomes. Mutations in genes for telomere binding proteins or components of telomerase give rise to the premature aging disorder dyskeratosis congenita (DC), which is characterized by extremely short telomeres and an aging phenotype. The current study demonstrates that DC cells signal a DNA damage response through p53 and its downstream mediator, p21WAF/CIP, which is accompanied by an elevation in steady-state levels of superoxide and percent glutathione disulfide, both indicators of oxidative stress. Poor proliferation of DC cells can be partially overcome by reducing O2tension from 21% to 4%. Further, restoring telomerase activity or inhibiting p53 or p21WAF/CIPsignificantly mitigated growth inhibition as well as caused a significant decrease in steady-state levels of superoxide. Our results support a model in which telomerase insufficiency in DC leads to p21WAF/CIPsignaling, via p53, to cause increased steady-state levels of superoxide, metabolic oxidative stress, and senescence. Copyright 2011, Mary Ann Liebert, Inc.
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    Digital Object Identifier (doi)

    Author List

  • Westin ER; Aykin-Burns N; Buckingham EM; Spitz DR; Goldman FD; Klingelhutz AJ
  • Start Page

  • 985
  • End Page

  • 997
  • Volume

  • 14
  • Issue

  • 6