A neuroprotective role for angiogenin in models of Parkinson's disease.

Academic Article

Abstract

  • We previously observed marked down-regulation of the mRNA for angiogenin, a potent inducer of neovascularization, in a mouse model of Parkinson's disease (PD) based on over-expression of alpha-synuclein. Angiogenin has also been recently implicated in the pathogenesis of amyotrophic lateral sclerosis. In this study, we confirmed that mouse angiogenin-1 protein is dramatically reduced in this transgenic alpha-synuclein mouse model of PD, and examined the effect of angiogenin in cellular models of PD. We found that endogenous angiogenin is present in two dopamine-producing neuroblastoma cell lines, SH-SY5Y and M17, and that exogenous angiogenin is taken up by these cells and leads to phosphorylation of Akt. Applied angiogenin protects against the cell death induced by the neurotoxins 1-methyl-4-phenylpyridinium and rotenone and reduces the activation of caspase 3. Together our data supports the importance of angiogenin in protecting against dopaminergic neuronal cell death and suggests its potential as a therapy for PD.
  • Published In

    Keywords

  • 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine, Animals, Cell Death, Cell Line, Tumor, Cytoprotection, Disease Models, Animal, Dopamine, Humans, Mice, Mice, Knockout, Mice, Transgenic, Nerve Degeneration, Neuroblastoma, Neuroprotective Agents, Oncogene Protein v-akt, Parkinsonian Disorders, Phosphorylation, Ribonuclease, Pancreatic
  • Digital Object Identifier (doi)

    Author List

  • Steidinger TU; Standaert DG; Yacoubian TA
  • Start Page

  • 334
  • End Page

  • 341
  • Volume

  • 116
  • Issue

  • 3