© 2015 Elsevier B.V. Background: Manual lesion delineation by an expert is the standard for lesion identification in MRI scans, but it is time-consuming and can introduce subjective bias. Alternative methods often require multi-modal MRI data, user interaction, scans from a control population, and/or arbitrary statistical thresholding. New method: We present an approach for automatically identifying stroke lesions in individual T1-weighted MRI scans using naïve Bayes classification. Probabilistic tissue segmentation and image algebra were used to create feature maps encoding information about missing and abnormal tissue. Leave-one-case-out training and cross-validation was used to obtain out-of-sample predictions for each of 30 cases with left hemisphere stroke lesions. Results: Our method correctly predicted lesion locations for 30/30 un-trained cases. Post-processing with smoothing (8. mm FWHM) and cluster-extent thresholding (100 voxels) was found to improve performance. Comparison with existing method: Quantitative evaluations of post-processed out-of-sample predictions on 30 cases revealed high spatial overlap (mean Dice similarity coefficient. = 0.66) and volume agreement (mean percent volume difference. = 28.91; Pearson's r= 0.97) with manual lesion delineations. Conclusions: Our automated approach agrees with manual tracing. It provides an alternative to automated methods that require multi-modal MRI data, additional control scans, or user interaction to achieve optimal performance. Our fully trained classifier has applications in neuroimaging and clinical contexts.