Parvovirus B19-specific DNA in bone marrow from B19 arthropathy patients: evidence for B19 virus persistence.

Academic Article

Abstract

  • To determine if B19 infection persists in patients with chronic B19 arthropathy, acute B19 infection in adults was documented by IgM serology. Bone marrow aspirates were obtained 24-42 months after acute infection in 4 patients who developed chronic joint symptoms. DNA from bone marrow aspirates was amplified by polymerase chain reaction by using B19-specific DNA sequences in the viral capsid gene and probing with B19-specific oligonucleotides in Southern analysis. B19-specific DNA sequences were detected in all 4 chronic arthropathy patients compared with 0 of 7 anti-B19 IgM-, IgG- and 0 of 6 anti-B19 IgM-, IgG+ normal bone marrow donors. Three patients with serologically proven acute B19 infection and acute but nonchronic joint symptoms had B19 DNA detected in bone marrow aspirates 2-18 months after infection. These findings suggest that B19 arthropathy is associated with persistence of either B19 virus or select B19 DNA sequences.
  • Authors

    Published In

    Keywords

  • Arthritis, Infectious, Base Sequence, Blotting, Southern, Bone Marrow, Chronic Disease, DNA, Viral, Erythema Infectiosum, Humans, Molecular Sequence Data, Parvovirus B19, Human, Polymerase Chain Reaction, Sensitivity and Specificity
  • Author List

  • Foto F; Saag KG; Scharosch LL; Howard EJ; Naides SJ
  • Start Page

  • 744
  • End Page

  • 748
  • Volume

  • 167
  • Issue

  • 3