B lymphocytes are required during the early priming of CD4+ T cells for clearance of pneumocystis infection in mice

Academic Article

Abstract

  • © 2015 by The American Association of Immunologists, Inc. B cells play a critical role in the clearance of Pneumocystis. In addition to production of Pneumocystis-specific Abs, B cells are required during the priming phase for CD4+ T cells to expand normally and generate memory. Clearance of Pneumocystis was found to be dependent on Ag specific B cells and on the ability of B cells to secrete Pneumocystis-specific Ab, as mice with B cells defective in these functions or with a restricted BCR were unable to control Pneumocystis infection. Because Pneumocystis-specific antiserum was only able to partially protect B cell-deficient mice from infection, we hypothesized that optimal T cell priming requires fully functional B cells. Using adoptive transfer and B cell depletion strategies, we determined that optimal priming of CD4+ T cells requires B cells during the first 2-3 d of infection and that this was independent of the production of Ab. T cells that were removed from Pneumocystis-infected mice during the priming phase were fully functional and able to clear Pneumocystis infection upon adoptive transfer into Rag1-/- hosts, but this effect was ablated in mice that lacked fully functional B cells. Our results indicate that T cell priming requires a complete environment of Ag presentation and activation signals to become fully functional in this model of Pneumocystis infection.
  • Published In

    Digital Object Identifier (doi)

    Pubmed Id

  • 1310206
  • Author List

  • Opata MM; Hollifield ML; Lund FE; Randall TD; Dunn R; Garvy BA; Feola DJ
  • Start Page

  • 611
  • End Page

  • 620
  • Volume

  • 195
  • Issue

  • 2