Long-term treatment of the MRL/lpr mouse with methotrexate and 10-deazaaminopterin

Academic Article

Abstract

  • Female MRL/lpr mice were treated with I.P. doses of methotrexate (MTX) and 10-deazaaminopterin (DAAM) in the range of 1 to 100 mg/kg body weight/week, in two equally divided doses. Treatment began at 7 weeks of age and continued to 30 weeks of age. Joint histopathology scores were tightly correlated with skin lesion-proteinuria scores at 30 weeks of age. MTX at levels of 5, 25, and 100 mg/kg body weight/week and DAAM at a level of 25 mg/kg body weight/week significantly reduced skin lesion-proteinuria scores below controls in a dose dependent manner. Animals receiving MTX at 25 mg/kg body weight/week had a significantly longer median life span and animals receiving MTX at 100 mg/kg body weight/week had a greater than 15% suppression of growth when compared with cottrols. Longevity and skin lesion-proteinuria scores appeared to be good indicators of drug efficacy while growth suppression appeared to be a good indicator of drug toxicity. ¬© 1992 Birkh√§user Verlag.
  • Digital Object Identifier (doi)

    Author List

  • Baggott JE; Morgan SL; Freeberg LE; Hudson BB; Vaughn WH; Gopal Nair M; Krumdieck CL; Koopman WJ; Gay RE; Gay S
  • Start Page

  • 104
  • End Page

  • 111
  • Volume

  • 35
  • Issue

  • 1-2