Increased expression of activation-induced cytidine deaminase is associated with anti-CCP and rheumatoid factor in rheumatoid arthritis.

Academic Article

Abstract

  • Rheumatoid arthritis (RA) is associated with higher levels of autoantibodies and IL-17. Here, we investigated if ectopic lymphoid follicles and peripheral blood mononuclear cells (PBMCs) from RA patients exhibit increased activation-induced cytidine deaminase (AID), and if increased AID is correlated with serum levels of autoantibodies and IL-17. The results of immunohistochemical staining showed that organized AID(+) germinal centres were observed in six of the 12 RA synovial samples, and AID(+) cells were found almost exclusively in the B-cell areas of these follicles. Aggregated but not organized lymphoid follicles were found in only one OA synovial sample without AID(+) cells. Significantly higher levels of AID mRNA (Aicda) detected by RT-PCR were found in the PBMCs from RA patients than PBMCs from normal controls (P < 0.01). In the PBMCs from RA patients, AID was expressed predominately by the CD10(+)IgM(+)CD20(+) B-cell population and the percentage of these cells that expressed AID was significantly higher than in normal controls (P < 0.01). AID expression in the PBMCs correlated significantly and positively with the serum levels of rheumatoid factor (RF) (P
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    Keywords

  • Aged, Arthritis, Rheumatoid, Autoantibodies, B-Lymphocytes, Cytidine Deaminase, Female, Germinal Center, Humans, Interferon-gamma, Interleukin-17, Male, Middle Aged, Peptides, Cyclic, Rheumatoid Factor, T-Lymphocytes, Helper-Inducer
  • Digital Object Identifier (doi)

    Pubmed Id

  • 7833292
  • Author List

  • Xu X; Hsu H-C; Chen J; Grizzle WE; Chatham WW; Stockard CR; Wu Q; Yang PA; Holers VM; Mountz JD
  • Start Page

  • 309
  • End Page

  • 316
  • Volume

  • 70
  • Issue

  • 3