Differential protein expression profiling by iTRAQ-2DLC-MS/MS of lung cancer cells undergoing epithelial-mesenchymal transition reveals a migratory/invasive phenotype

Academic Article

Abstract

  • Transforming growth factor-β (TGF-β) induces epithelial- mesenchymal transition (EMT) of epithelial cells in both normal embryonic development and certain pathological contexts. Here, we show that TGF-β induced-EMT in human lung cancer cells (A549; adenocarcinoma cells) mediates tumor cell migration and invasion phenotypes. To gain insights into molecular events during EMT, we employed a global stable isotope labeled profiling strategy using iTRAQ reagents, followed by 2DLC-MS/MS, which identified a total of 51 differentially expressed proteins during EMT; 29 proteins were up-regulated and 22 proteins were down-regulated. Down-regulated proteins were predominantly enzymes involved in regulating nutrient or drug metabolism. The majority of the TGF-β-induced proteins (such as tropomyosins, filamin A, B, & C, integrin-β1, heat shock protein27, transglutaminase2, cofilin, 14-3-3 zeta, ezrin-radixin-moesin) are involved in the regulation of cell migration, adhesion and invasion, suggesting the acquisition of a invasive phenotype. © 2006 American Chemical Society.
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    Digital Object Identifier (doi)

    Author List

  • Keshamouni VG; Michailidis G; Grasso CS; Anthwal S; Strahler JR; Walker A; Arenberg DA; Reddy RC; Akulapalli S; Thannickal VJ
  • Start Page

  • 1143
  • End Page

  • 1154
  • Volume

  • 5
  • Issue

  • 5