Novel gene therapy strategy to accomplish growth factor modulation induces enhanced tumor cell chemosensitivity

Academic Article

Abstract

  • erbB-2 is a cell surface transmembrane glycoprotein which, when overexpressed, has been shown to be relevant to intrinsic tumor cell chemoresistance. Thus, strategies to down-regulate cell surface erbB-2 have resulted in enhanced tumor cell chemosensitivity. We have recently reported a gene therapy strategy to down-modulate erbB-2 expression using a plasmid construct encoding an intracellular single chain antibody. Therefore, we now demonstrate enhanced chemosensitivity to cis-diamminedichloroplatinum in erbB-2 overexpressing tumor cells and a model system of stable clones using an intracellular single chain antibody. These findings are consistent with the hypothesis that erbB-2 plays a role in tumor cell chemoresistance. In addition, these findings represent a novel gene therapy approach to overcome erbB-2 mediated tumor cell chemoresistance.
  • Published In

    Author List

  • Barnes MN; Deshane JS; Siegal GP; Alvarez RD; Curiel DT
  • Start Page

  • 1089
  • End Page

  • 1095
  • Volume

  • 2
  • Issue

  • 7