Neutrophils contain prolyl endopeptidase and generate the chemotactic peptide, PGP, from collagen.

Academic Article

Abstract

  • Prolyl endopeptidase (PE), a protease that cleaves after proline residues in oligopeptides, is highly active in brain and degrades neuropeptides in vitro. We have recently demonstrated that PE, in concert with MMP's, can generate PGP (proline-glycine-proline), a novel, neutrophil chemoattractant, from collagen. In this study, we demonstrate that human peripheral blood neutrophils contain PE, which is constitutively active, and can generate PGP de novo from collagen after activation with LPS. This novel, pro-inflammatory role for PE raises the possibility of a self-sustaining pathway of neutrophilic inflammation and may provide biomarkers and therapeutic targets for diseases caused by chronic, neutrophilic inflammation.
  • Published In

    Keywords

  • Adjuvants, Immunologic, Chromatography, Liquid, Collagen, Humans, Lipopolysaccharides, Neutrophil Activation, Neutrophils, Oligopeptides, Proline, Serine Endopeptidases, Tandem Mass Spectrometry
  • Digital Object Identifier (doi)

    Author List

  • O'Reilly PJ; Hardison MT; Jackson PL; Xu X; Snelgrove RJ; Gaggar A; Galin FS; Blalock JE
  • Start Page

  • 51
  • End Page

  • 54
  • Volume

  • 217
  • Issue

  • 1-2