Double-barrelled potassium selective microelectrodes (K-ISME) were used in situ for real-time sequential determinations of potassium concentrations (C(K+)) in endolymph, marginal cells and the spiral ligaments of rats exposed to moderate noise at 100 dB for 30 min (NE) and control (CTL) animals. C(K+) in NE animals at these sites did not differ significantly when compared to C(K+) in CTL animals. However, there was a slight decrease in C(K+) in marginal cells in the noise-exposed animals.